Established in 1963 by scientists George Todardo and Howard Green, the NIH3T3 cell line was derived from mouse embryonic fibroblasts. The cells spontaneously immortalized and were named after their culturing protocol, “3-day transfer, inoculum 3×105 cells”.

What are 3T3-L1 cells?

3T3-L1 is a cell line derived from (mouse) 3T3 cells that is used in biological research on adipose tissue. 3T3-L1 cells have a fibroblast-like morphology, but, under appropriate conditions, the cells differentiate into an adipocyte-like phenotype.

What are 3T3 fibroblasts?

3T3 cells are several cell lines of mouse embryonic fibroblasts. The original 3T3 cell line (3T3-Swiss albino) was established in 1962 by two scientists then at the Department of Pathology in the New York University School of Medicine, George Todaro and Howard Green.

What is NIH 3T3 cell line?

NIH 3T3 Cells. A continuous cell line of high contact-inhibition established from NIH Swiss mouse embryo cultures. The cells are useful for DNA transfection and transformation studies. (From ATCC [Internet]. Virginia: American Type Culture Collection; c2002 [cited 2002 Sept 26]. Available from )

Where can I buy NIH/3T3 transfection reagent?

Commercially available NIH/3T3 transfection reagent is available from Altogen Biosystems • Cytogenic characterization of the cell line: In this study, scientists studied the genome of NIH 3T3 cells.

What is the confluent density of NIH3T3 cells?

NIH 3T3 Cells. Cells become confluent at a density of approximately 40,000 cells/cm 2, and a saturation density of about 50,000 cells can be reached. However, in culture, cells should not be allowed to become completely confluent as this results in cells becoming senescent. NIH3T3 cells have a doubling rate of 20-26 hours…

Can NIH3T3 cells be cultured in DMEM?

NIH 3T3 Cells. NIH3T3 cells have a doubling rate of 20-26 hours and should be cultured in a plastic flask (i.e. they do not adhere well to certain types of glass). The common medium used for culturing NIH3T3 cells is Dulbecco’s Modified Eagle Medium (DMEM) formulated with the addition of 10% FBS. Flasks should be incubated at 37°C in 5% CO2.